Part:BBa_K1736000:Design

Harmonised B. subtilis Falvin Binding Fluorescent Protein (BsFP)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 109
  Illegal BamHI site found at 229
  Illegal XhoI site found at 425
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The BsFP protein was harmonised by the method outlined in Angov et al. ¹ for which we utilised translation rate quantisations proposed by Spencer et al. ². To download and obtain instructions on how to use the algorithm to harmonise your sequence, refer to the Sydney 2015 [http://2015.igem.org/Team:Sydney_Australia/Modeling modelling page]. Note that the part also contains an B.subtilis RBS.

Source

Bacillus Subtilits

References

¹ Angov E et al., 2008, “Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host”, PLoS One, Vol 3.

² Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.